Provinase® is a powerful, non-specific endonuclease enzyme that cleaves both DNA and RNA—regardless of sequence—into small oligonucleotides. Its mechanism is designed to efficiently degrade contaminating nucleic acids in a wide range of biological and bioprocessing applications.

Mechanism of Action:

1. Nucleic Acid Binding
   Provinase® identifies and binds to exposed double-stranded or single-stranded DNA and RNA present in a solution, such as those released from host cells during bioproduction or sample preparation.
2. Phosphodiester Bond Hydrolysis
   It then catalyzes the hydrolysis of phosphodiester bonds within the nucleic acid backbone, fragmenting long DNA/RNA strands into short oligonucleotides or mononucleotides.
3. Rapid and Non-Sequence-Specific Cleavage
   Unlike restriction enzymes, Provinase® does not require a specific recognition sequence, making it effective across diverse sample types and species. It acts quickly and uniformly across nucleic acid types.
4. End Product
   The degraded nucleic acids are rendered biologically inactive and are easily removed via filtration, chromatography, or centrifugation, contributing to lower viscosity, higher purity, and more efficient downstream processing.

   
   Key Features of Provinase® Action:

• Works on both DNA and RNA
• Non-sequence-specific, enabling broad utility
• Functions under a wide range of pH and buffer conditions
• Preserves the integrity of target proteins, viruses, or therapeutic vectors

This makes Provinase® an indispensable tool in gene therapy, vaccine production, diagnostic workflows, and research, where removal of unwanted nucleic acids is critical for safety, performance, and regulatory compliance.